id_851. NATURAL ANTIOXIDANTS 7,8-DIHYDROXYFLAVONE AND NARINGIN AS POTENTIAL THERAPEUTIC AGENTS IN EXPERIMENTAL DEPRESSION
Klimentova Jana1, Ondicova Katarina1,2, Vrankova Stanislava1,3
1 Centre of Experimental Medicine, Slovak Academy of Sciences, Institute of Normal and Pathological Physiology, Sienkiewiczova 1, Bratislava, Slovakia
2 Biomedical Research Center, Slovak Academy of Sciences, Institute of Experimental Endocrinology, Dubravska cesta 9, Bratislava, Slovakia
3 Faculty of Medicine, Comenius University, Institute of Pathophysiology, Spitalska 24, Bratislava. Slovakia
INTRODUCTION: Naringin and 7,8-dihydroxyflavone (7,8-DHF) are naturally occurring bioflavonoids, that exhibit antioxidant, anti-inflammatory and antidepressant effects. Concurrent administration of 7,8-dihydroxyflavone and Naringin may lead to a synergistic effect, improving NO production and redox status.
AIM(S): The aim of our study was to investigate the effects of 7,8-DHF and Naringin, as well as their combinations on neurobiological and behavioral changes in the model of depression.
METHOD(S): We used 12-week-old female Sprague Dawley rats (SD), which were exposed to unpredictable chronic mild stress (CUS) for 3 weeks. SD rats were divided into five groups: control gorup, CUS group, CUS + 7,8- DHF, CUS + Naringin and CUS + 7,8- DHF + Naringin. We used forced swim test (FST) and elevated plus maze (EPM) test to detect behavioral changes. NO-synthase (NOS) activity, protein expression, and concentration of conjugated dienes (CD) were measured.
RESULTS: We found higher immobility in CUS group in the FST. Both 7,8-DHF and Naringin decreased immobility and the combination was more effective. Rats that were given Naringin or the combination treatment spent more time in the open arms of the EPM and made more entries than the CUS group. NOS activity remained unchanged in the CUS group in medial prefrontal cortex (mPFC). The Naringin and combination of 7,8-DHF+Naringin had significant effect on increasing NOS activity. 7,8-DHF administration increased the expression of nNOS, BDNF, and SOD in the mPFC. The combination of 7,8-DHF+Naringin increased nNOS and SOD1 expression, while Naringin alone increased only SOD1 expression. The concentration of CD was increased after CUS, while 7,8-DHF and 7,8-DHF+Naringin reduced CD concentration.
CONCLUSIONS: Our findings suggested that both 7,8-DHF and Naringin have antidepressant and antioxidant properties, and that combination therapy provides stronger behavioural benefits against changes induced by chronic unpredictable stress.
FINANCIAL SUPPORT: Supported by grant VEGA 2/0122/24