id_932. IDENTIFICATION OF FACTORS STIMULATING CORNEAL WOUND HEALING
Anna Pacwa1,2,3, Bartosz Machna1,4,5, Klaudia Mróz1,4, Anna Gąsiorek1,4, Monika Katan1,4, Maciej Osęka5, Joanna Lewin-Kowalik1,2,4, Adrian Smędowski1,2,4,6,7
1 The Laboratory for Translational Research in Ophthalmology, Department of Ophthalmology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland
2 Glaucotech Co, Katowice, Poland
3 Department of Clinical Genetics and Rare Diseases, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Katowice, Poland
4 Department of Ophthalmology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland
5 Oftalabs, Wałbrzych, Poland
6 Department of Ophthalmology, Professor K. Gibinski University Clinical Center, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland
7 Department of Paedriatric Ophthalmology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland
INTRODUCTION: Corneal wound healing is a complex process involving epithelial cells recovery and tissue integrity restoration. Disruptions in this process can cause permanent damage and impaired vision. Currently, intensive research is being conducted on substances that support corneal regeneration.
AIM(S): The aim of this study was to determine a safe dose of D-Panthenol, Cabergoline and Pergolide to evaluate their effects on the wound healing process in cultures of human corneal epithelial cells (HCE-2).
METHOD(S): HCE cells were exposed to increasing concentrations of D-Panthenol (0.5-15%), Cabergoline (0.001-100 µM) and Pergolide (0.25-2 µg/mL). After 6, 12, 24 and 48 hours of incubation, a cell viability assay (Alamar Blue) and a cytotoxicity assay (RealTime-Glo) were performed. The positive control was an increasing concentration of H2O2 and the negative control was cells in culture medium. Based on the results, the optimal doses of the test substances were selected: D-Panthenol 1%, Cabergoline 100 µM, Pergolide 1 µg/mL to assess wound healing, which was carried out in Ibidi Culture-Insert 2 Well in µ-Dish 35 mm chambers. To assess cell proliferation and migration, microscopic images were taken of the cell culture exposed to the test substances at different time points.
RESULTS: Based on the tests performed, safe doses of the substances were established: D-Panthenol 1%, Cabergoline 100 µM and Pergolide 1 µg/mL. Analysis of microscopic images showed increased proliferation and migration of HCE cells compared to controls after exposure to Pergolide at a concentration of 1 µg/mL. The other supplements had no effect on HCE migration
CONCLUSIONS: The results showed that Pergolide may be a promising candidate for future regenerative therapies, especially in the context of treating corneal epithelial damage.
FINANCIAL SUPPORT: This project was conducted in collaboration with Oftalabs